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The primary goal of this study was to model the soil emissions during camelina and wheat production in a three-year cycle in the Pacific North West region of the United and to evaluate the effect of regional agro-climatic variations on the life cycle impact of biofuel production from camelina. The model was run for 60 years to reach to the soil organic carbon (SOC) equilibrium point. The results from the cycle with the SOC equilibrium were used to conduct life cycle assessment (LCA). Energy allocation method was selected to allocate LCA results based on the energy content of products and co-products. The results showed that no-tillage practice had lower GHG emissions compared to conventional tillage system and locations with income equal or more than the break-even cost and low environmental impacts are suitable for the winter wheat-fallow camelina rotation system.