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Summary: It is desirable to increase the content of the monounsaturated oleic acid and decrease the content of the polyunsaturated fatty acids, linoleic acid, and α-linolenic acids in camelina oils to improve oxidative stability. Desaturation of oleic acid to polyunsaturated acids is mainly controlled by the gene FAD2. Three FAD2 genes were identified in Camelina, with very high sequence similarity but different expression patterns. The introns of the three CsFAD2 genes, which showed greater sequence variations, may provide additional resources for designing molecular markers in breeding.